Variable Nitrogen Fixation in Wild Populus.
Identifieur interne : 001606 ( Main/Exploration ); précédent : 001605; suivant : 001607Variable Nitrogen Fixation in Wild Populus.
Auteurs : Sharon L. Doty [États-Unis] ; Andrew W. Sher [États-Unis] ; Neil D. Fleck [États-Unis] ; Mahsa Khorasani [États-Unis] ; Roger E. Bumgarner [États-Unis] ; Zareen Khan [États-Unis] ; Andrew W K. Ko [États-Unis] ; Soo-Hyung Kim [États-Unis] ; Thomas H. Deluca [États-Unis]Source :
- PloS one [ 1932-6203 ] ; 2016.
Descripteurs français
- KwdFr :
- MESH :
- microbiologie : Populus.
- métabolisme : Populus.
- Fixation de l'azote, Microbiote.
English descriptors
- KwdEn :
- MESH :
- metabolism : Populus.
- microbiology : Populus.
- Microbiota, Nitrogen Fixation.
Abstract
The microbiome of plants is diverse, and like that of animals, is important for overall health and nutrient acquisition. In legumes and actinorhizal plants, a portion of essential nitrogen (N) is obtained through symbiosis with nodule-inhabiting, N2-fixing microorganisms. However, a variety of non-nodulating plant species can also thrive in natural, low-N settings. Some of these species may rely on endophytes, microorganisms that live within plants, to fix N2 gas into usable forms. Here we report the first direct evidence of N2 fixation in the early successional wild tree, Populus trichocarpa, a non-leguminous tree, from its native riparian habitat. In order to measure N2 fixation, surface-sterilized cuttings of wild poplar were assayed using both 15N2 incorporation and the commonly used acetylene reduction assay. The 15N label was incorporated at high levels in a subset of cuttings, suggesting a high level of N-fixation. Similarly, acetylene was reduced to ethylene in some samples. The microbiota of the cuttings was highly variable, both in numbers of cultured bacteria and in genetic diversity. Our results indicated that associative N2-fixation occurred within wild poplar and that a non-uniformity in the distribution of endophytic bacteria may explain the variability in N-fixation activity. These results point to the need for molecular studies to decipher the required microbial consortia and conditions for effective endophytic N2-fixation in trees.
DOI: 10.1371/journal.pone.0155979
PubMed: 27196608
PubMed Central: PMC4873266
Affiliations:
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Ko</name><affiliation wicri:level="4"><nlm:affiliation>School of Environmental and Forest Sciences, College of the Environment, University of Washington, Seattle, Washington, United States of America.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>School of Environmental and Forest Sciences, College of the Environment, University of Washington, Seattle, Washington</wicri:regionArea><placeName><region type="state">Washington (État)</region><settlement type="city">Seattle</settlement></placeName><orgName type="university">Université de Washington</orgName></affiliation></author><author><name sortKey="Kim, Soo Hyung" sort="Kim, Soo Hyung" uniqKey="Kim S" first="Soo-Hyung" last="Kim">Soo-Hyung Kim</name><affiliation wicri:level="4"><nlm:affiliation>School of Environmental and Forest Sciences, College of the Environment, University of Washington, Seattle, Washington, United States of America.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>School of Environmental and Forest Sciences, College of the Environment, University of Washington, Seattle, Washington</wicri:regionArea><placeName><region type="state">Washington (État)</region><settlement type="city">Seattle</settlement></placeName><orgName type="university">Université de Washington</orgName></affiliation></author><author><name sortKey="Deluca, Thomas H" sort="Deluca, Thomas H" uniqKey="Deluca T" first="Thomas H" last="Deluca">Thomas H. Deluca</name><affiliation wicri:level="4"><nlm:affiliation>School of Environmental and Forest Sciences, College of the Environment, University of Washington, Seattle, Washington, United States of America.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>School of Environmental and Forest Sciences, College of the Environment, University of Washington, Seattle, Washington</wicri:regionArea><placeName><region type="state">Washington (État)</region><settlement type="city">Seattle</settlement></placeName><orgName type="university">Université de Washington</orgName></affiliation></author></analytic><series><title level="j">PloS one</title><idno type="eISSN">1932-6203</idno><imprint><date when="2016" type="published">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Microbiota (MeSH)</term><term>Nitrogen Fixation (MeSH)</term><term>Populus (metabolism)</term><term>Populus (microbiology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Fixation de l'azote (MeSH)</term><term>Microbiote (MeSH)</term><term>Populus (microbiologie)</term><term>Populus (métabolisme)</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Populus</term></keywords><keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Populus</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Populus</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Populus</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Microbiota</term><term>Nitrogen Fixation</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Fixation de l'azote</term><term>Microbiote</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The microbiome of plants is diverse, and like that of animals, is important for overall health and nutrient acquisition. In legumes and actinorhizal plants, a portion of essential nitrogen (N) is obtained through symbiosis with nodule-inhabiting, N2-fixing microorganisms. However, a variety of non-nodulating plant species can also thrive in natural, low-N settings. Some of these species may rely on endophytes, microorganisms that live within plants, to fix N2 gas into usable forms. Here we report the first direct evidence of N2 fixation in the early successional wild tree, Populus trichocarpa, a non-leguminous tree, from its native riparian habitat. In order to measure N2 fixation, surface-sterilized cuttings of wild poplar were assayed using both 15N2 incorporation and the commonly used acetylene reduction assay. The 15N label was incorporated at high levels in a subset of cuttings, suggesting a high level of N-fixation. Similarly, acetylene was reduced to ethylene in some samples. The microbiota of the cuttings was highly variable, both in numbers of cultured bacteria and in genetic diversity. Our results indicated that associative N2-fixation occurred within wild poplar and that a non-uniformity in the distribution of endophytic bacteria may explain the variability in N-fixation activity. These results point to the need for molecular studies to decipher the required microbial consortia and conditions for effective endophytic N2-fixation in trees.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">27196608</PMID><DateCompleted><Year>2017</Year><Month>07</Month><Day>07</Day></DateCompleted><DateRevised><Year>2019</Year><Month>02</Month><Day>13</Day></DateRevised><Article PubModel="Electronic-eCollection"><Journal><ISSN IssnType="Electronic">1932-6203</ISSN><JournalIssue CitedMedium="Internet"><Volume>11</Volume><Issue>5</Issue><PubDate><Year>2016</Year></PubDate></JournalIssue><Title>PloS one</Title><ISOAbbreviation>PLoS One</ISOAbbreviation></Journal><ArticleTitle>Variable Nitrogen Fixation in Wild Populus.</ArticleTitle><Pagination><MedlinePgn>e0155979</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1371/journal.pone.0155979</ELocationID><Abstract><AbstractText>The microbiome of plants is diverse, and like that of animals, is important for overall health and nutrient acquisition. In legumes and actinorhizal plants, a portion of essential nitrogen (N) is obtained through symbiosis with nodule-inhabiting, N2-fixing microorganisms. However, a variety of non-nodulating plant species can also thrive in natural, low-N settings. Some of these species may rely on endophytes, microorganisms that live within plants, to fix N2 gas into usable forms. Here we report the first direct evidence of N2 fixation in the early successional wild tree, Populus trichocarpa, a non-leguminous tree, from its native riparian habitat. In order to measure N2 fixation, surface-sterilized cuttings of wild poplar were assayed using both 15N2 incorporation and the commonly used acetylene reduction assay. The 15N label was incorporated at high levels in a subset of cuttings, suggesting a high level of N-fixation. Similarly, acetylene was reduced to ethylene in some samples. The microbiota of the cuttings was highly variable, both in numbers of cultured bacteria and in genetic diversity. Our results indicated that associative N2-fixation occurred within wild poplar and that a non-uniformity in the distribution of endophytic bacteria may explain the variability in N-fixation activity. These results point to the need for molecular studies to decipher the required microbial consortia and conditions for effective endophytic N2-fixation in trees.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Doty</LastName><ForeName>Sharon L</ForeName><Initials>SL</Initials><AffiliationInfo><Affiliation>School of Environmental and Forest Sciences, College of the Environment, University of Washington, Seattle, Washington, United States of America.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Sher</LastName><ForeName>Andrew W</ForeName><Initials>AW</Initials><AffiliationInfo><Affiliation>School of Environmental and Forest Sciences, College of the Environment, University of Washington, Seattle, Washington, United States of America.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Fleck</LastName><ForeName>Neil D</ForeName><Initials>ND</Initials><AffiliationInfo><Affiliation>School of Environmental and Forest Sciences, College of the Environment, University of Washington, Seattle, Washington, United States of America.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Khorasani</LastName><ForeName>Mahsa</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>School of Environmental and Forest Sciences, College of the Environment, University of Washington, Seattle, Washington, United States of America.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Bumgarner</LastName><ForeName>Roger E</ForeName><Initials>RE</Initials><AffiliationInfo><Affiliation>Department of Microbiology, University of Washington, Seattle, Washington, United States of America.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Khan</LastName><ForeName>Zareen</ForeName><Initials>Z</Initials><AffiliationInfo><Affiliation>School of Environmental and Forest Sciences, College of the Environment, University of Washington, Seattle, Washington, United States of America.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ko</LastName><ForeName>Andrew W K</ForeName><Initials>AW</Initials><AffiliationInfo><Affiliation>School of Environmental and Forest Sciences, College of the Environment, University of Washington, Seattle, Washington, United States of America.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kim</LastName><ForeName>Soo-Hyung</ForeName><Initials>SH</Initials><AffiliationInfo><Affiliation>School of Environmental and Forest Sciences, College of the Environment, University of Washington, Seattle, Washington, United States of America.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>DeLuca</LastName><ForeName>Thomas H</ForeName><Initials>TH</Initials><AffiliationInfo><Affiliation>School of Environmental and Forest Sciences, College of the Environment, University of Washington, Seattle, Washington, United States of America.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType><PublicationType UI="D013486">Research Support, U.S. Gov't, Non-P.H.S.</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2016</Year><Month>05</Month><Day>19</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>PLoS One</MedlineTA><NlmUniqueID>101285081</NlmUniqueID><ISSNLinking>1932-6203</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D064307" MajorTopicYN="Y">Microbiota</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D009586" MajorTopicYN="Y">Nitrogen Fixation</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2015</Year><Month>12</Month><Day>16</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2016</Year><Month>05</Month><Day>06</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2016</Year><Month>5</Month><Day>20</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2016</Year><Month>5</Month><Day>20</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2017</Year><Month>7</Month><Day>8</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>epublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">27196608</ArticleId><ArticleId IdType="doi">10.1371/journal.pone.0155979</ArticleId><ArticleId 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